openalexAdvanced Science2026-07-23
Systematic Position Mapping of Split CRISPR‐Cas12a Activators Enables Highly Sensitive miRNA Detection and Cancer Cell Stratification
Xiaoyan Tang, Zhe Li, Yuning Lu, Ma My, Zijian Mo, Jiajun Ke, et al.
Amplification-free Cas12a diagnostics with split crRNA enable rapid and programmable target recognition, yet insufficient understanding of DNA activator architecture prevents predictable control over trans-cleavage activity and sensitivity. Here we systematically map over 200 spl…