Simultaneous determiantion of aripiprazole and escitalopram oxalate by HPLC

Divyakumari Kiransinh Solanki, Sagarkumar P. Patel, Jasmina Surati, Ashok H Akabari, Ketan Shah, Dipansu Sahu, Sagarkumar Patel

Simple, accurate, precise, and sensitive reverse-phase high-performance liquid chromatographic (HPLC) method was developed for the simultaneous measurement of ARI and ESC in pharmaceutical products by using a C18 column with dimensions of 250 mm x 4.6 mm and a particle size of 5 m.The mobile phase consisted of a mixture of acetonitrile, methanol, and water in the ratio of 80:05:15 v/v/v.Orthophosphoric acid was added to adjust the pH to 7.00 0.1.The detection wavelength used for measuring the absorbance of the components was 246 nm, and the Mobile Phase flow rate was set at 1.0 mL/min.The retention times of ARI and ESC were 5.63 and 3.65 minutes, respectively.The ARI and ESC correlation coefficients (R 2 -values) were observed at 0.9995 and 0.9997, respectively, demonstrating a robust linear relationship between the analyte concentration and the observed absorbance.The linearity range for ARI was 20 to 120 g/mL, while ESC was 60 to 360 g/mL.The recovery percentages for ARI and ESC ranged from 99.43% to 100.65% and 99.58% to 100.15%, respectively.Additionally, all parameter uncertainty levels were below 2. The method was validated following ICH guideline Q2 (R1).The analytical method validated will be successfully applied to the pharmaceutical dosage form.