openalexNature Chemical Biology2026-07-23
Using enantioselective biosensors to evolve asymmetric biocatalysts
Simon d’Oelsnitz, Wantae Kim, Nicole N Zhao, Haley A. Hardtke, Svetlana P. Ikonomova, Nina Alperovich, et al.
Biocatalysts are prized for their enantioselectivity, but slow chromatographic separations required to measure enantiomeric excess bottleneck their development. To overcome this limitation, we evolve enantioselective transcription factors (eTFs) that convert enzyme-catalyzed enan…