SeekBrain: An Autonomous Multi-Agent System for Accelerating Neuroscience Discovery

Jiamin Wu, Peishan Xiang, Jingyang Chen, Yuqing Zhu, Yuxi Li, Ling Luo, Qihao Zheng, Jialiang Zu, Yongchao Wu, Mindong Liu, Haitao Wu, Chaofan Hu, Yijie Sun, Yuqi Hang, Yu Zhu, Shuo Li, Yue Fan, Shiyang Feng, Wanghan Xu, Tianlei Zhang, Jie Zhang, Wenlong Zhang, Bo Zhang, Kai Wang, Lei Bai, Mianxin Liu, Wanli Ouyang, Jiulin Du, Chunfeng Song

Modern neuroscience relies on integrating multi-scale, multimodal datasets to uncover the neural principles underlying intelligence. However, analytical challenges posed by highly heterogeneous data and fragmented workflows increasingly constrain discoveries. Here we introduce SeekBrain, an autonomous multi-agent framework designed to accelerate neuroscience discovery through domain-grounded hierarchical planning and cross-modal data analysis. SeekBrain dynamically constructs a repertoire of analysis recipes extracted from code-paper pairs. By coupling this codified expertise with agentic planning and execution engines, the framework scalably generates hypotheses and analytical pipelines on demand. Systematic evaluation on the expert-annotated BrainArena benchmark demonstrates that SeekBrain substantially outperforms state-of-the-art agent baselines across various analysis tasks. Crucially, when deployed in real-world research, SeekBrain integrated behavioral, neural, and anatomical data to reveal structured, distributed neural representations of larval zebrafish behavior and a shared axis of regional decoding strength across the brain in a mouse decision-making task. These results establish SeekBrain as a scalable and practical tool for accelerating data-driven discoveries in neuroscience.